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Real-time queue length information is an important input for many traffic applications. This paper presents a novel method for real-time queue length detection with roadside LiDAR data. Vehicles on the road were continuously tracked with the LiDAR data processing procedures (including background filtering, point clustering, object classification, lane identification and object association). A detailed method to identify the vehicle at the end of the queue considering the occlusion issue and package loss issue was documented in this study