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5-27.5 and 16.9 g/kg total nitrogen (TN), respectively) were all significantly decreased in stylo silage. In addition, TA increased the relative abundance of Weissella, Acinetobacter, and Kosakonia spp. and decreased that of undesirable Clostridium spp. TA can thus be used to improve the silage quality of both mulberry leaf and stylo silage, with 2% TA being the better concentration of additive to use.Carbapenem-resistant Acinetobacter nosocomialis (CRAn) is a significant public health concern. Tigecycline non-susceptible CRAn (Tn-CRAn) i