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Ventilago denticulata is an herbal medicine for the treatment of wound disease; consequently this plant may abundant with antibacterial representatives. UHPLC-ESI-QTOF-MS/MS-Based molecular networking guided isolation and dereplication led to the identification of anti-bacterial and antifungal agents in V. denticulata. Nine antimicrobial representatives in V. denticulata were isolated and characterized; they have been divided into four teams including (I) flavonoid glycosides, rhamnazin 3-rhamninos