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To investigate the effects of Lactobacillus plantarum on inflammatory responses induced by ETEC K88 and explore the underlying molecular mechanisms. Intestinal porcine cells (IPEC-1) were incubated with 0 or 1×10 CFU per well L. plantarum for 4h, and then these cells were challenged with 0 or 1×10 CFU per well ETEC K88 for 2h. The results showed that pre-treatment of IPEC-1 cells with L. plantarum prevented the increases in the transcript abundance of interleukin-1α (IL-1α), interleukin-6 (IL-6), interleukin-8 (IL-8) and tumour necrosis f