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Assessment of amino acid bioavailability is of key importance for the evaluation of protein quality; however, measuring ileal digestibility of dietary proteins in humans is challenging. Therefore, a less-invasive dual stable isotope tracer approach was developed. We aimed to test the assumption that the 15N13C enrichment ratio in the blood increases proportionally to the quantity ingested by applying different quantities of 15N test protein. In a crossover design, 10 healthy adults were given a semi-liquid mixed meal containing 25 g (lo
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