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In this analysis, we discussed the reason why B. subtilis is an excellent chassis mobile for metabolic engineering. We also summarized the most recent research progress in systematic biology, artificial biology and evolution-based manufacturing of B. subtilis, and showed systemic metabolic engineering expedite the harnessing B. subtilis for bioproduction.The cytochrome P450 enzyme CYP-sb21 from the rare actinomycete Sebekia benihana is effective at hydroxylating the immunosuppressive drug
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