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More has to be investigated on the use of ozonated water (O3) for the improvement of growth medium properties. Accordingly, the objective was to examine the effects of O3 (control, 0.5, 1.0, and 2.0 mg L-1) on soil physicochemical (colloidal) properties using organic fertilization (manure), under non-planted or planted conditions. Different soil physicochemical (colloidal) properties including soil available water (SAW), aggregate stability, soil porosity, pH, salinity (EC), organic carbon (SOC), CaCO3, and cation exchange capacity (CEC)