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Blood values affected by parity in lactating dairy cows grazing in highland tropical herds included serum calcium levels, red blood cell counts, hematocrit, hemoglobin, mean corpuscular hemoglobin, and total eosinophil counts, whereas blood values affected by season included total leukocyte counts, total and differential lymphocyte counts, serum albumin and globulin concentration, and serum albuminglobulin ratio (P less then 0.05). Data from our study could be used for comparison studies between lactating dairy cows within tropical herds