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58 ± 18.6% vs. 6.01 ± 25.41%, P less then 0.0001, 79.83 ± 12.79% vs. 51.16 ± 13.62%, P less then 0.0001, respectively). The 24 h versus 2 h suppression ratio showed the best diagnostic accuracy at a cut-point of 44.46% in the training cohort, with a sensitivity of 95.00%, a specificity of 93.75%, and a PPV of 88.89%. The accuracy was confirmed in the validation cohort. Three out of twelve patients in the prospective cohort showed a TSH suppression ratio greater than 44.46% and all developed microadenomas during follow-up. Conclusions A
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