https://mirnaarray.com/multidr....ug-resistant-listeri
More, heat, pH, polyphosphate focus, and focus of divalent material ions for 3'-SL production were optimized. Finally, a simple yet effective biotransformation system had been established under the optimized conditions. The utmost production of 3'-SL reached 38.7 mM, and a molar yield of 97.1per cent from N-acetylneuraminic acid (NeuAc, sialic acid, SA) had been acquired. The outcomes display that the multi-enzymatic cascade biosynthetic path with cofactor regeneratio