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The ultrasonic-assisted alkali extraction of Typha domingensis stem polysaccharide (TDSPs) was studied using the response surface methodology. The optimal parameters of TDSPs with maximum yields (12.24± 0.08%) were as follows extraction time 40 min, NaOH concentration 1.5 M and the ratio of water to raw material 25mL/g. The experimental purity of TDSPs was 86.01 ± 0.02. Mineral elements were determined by ICP-AES. The gel permeation chromatography results indicated that TDSPs was a polysaccharide polymer with two peaks with molecular wei