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In this study, 20 PU-degrading strains had been separated, including 11 micro-organisms and 9 fungi, using a synthesized poly(1,4-butylene adipate)-based PU (PBA-PU) while the testing substrate. Three PU substrates with increasing framework complexities were utilized for an intensive evaluation of microbial degradation ability Impranil® DLN-SD, PBA-PU film and PU foam waste. After 4 days, the greatest fungal PBA-PU degrader, Cladosporium sp. P7, could break down 94.5% of Impranil® DLN-SD. After