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Background Congenital sensorineural deafness (CSD) is the most common type of deafness in Dalmatian dogs. Objectives To use results of CSD screening in Dalmatian dogs in the United Kingdom in genetic analysis and to determine any changes in the prevalence of CSD in this breed over time. Animals A total of 8955 Dalmatian puppies undergoing hearing function screening using brainstem auditory evoked response (BAER) between July 1992 and February 2019. Methods Results of BAER testing and pigmentation phenotypic data were linked to the UK Ken