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The role of host immune responses in the pathogenesis of borrelial dissemination in early Lyme borreliosis (L in the form of multiple erythema migrans (MEM) or LB-associated symptoms is incompletely understood. In this study, fifteen cytokine or chemokine levels, representative of innate, Th1, and Th17 immune responses, were assessed using a bead-based Luminex multiplex assay in acute sera from 76 adult patients with skin culture-positive Borrelia afzelii solitary erythema migrans (SEM) and 58 patients with MEM at a single-center univ