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To investigate the influence of topical cyclopentolate 1%, as an anti-muscarinic mydriatic agent, on the peripapillary and macular microvasculature by optical coherence tomography angiography (OCT-A) in healthy adults. A total of 41 healthy adults without any systemic or ocular disease were enrolled for this prospective consecutive study. All patients underwent OCT-A measurements (OptoVue Inc., Freemont, CA, USA) to assess optic disc status for radial peripapillary capillary network (whole image, inside disc, and peripapillary capillary