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A unique chromatography-based experimental method for deciding the configurations of stereogenic centers β to nitrogen atoms in macrolactams ended up being set up and successfully used in this report. These compounds exhibited considerable bioactivities contrary to the silkworm entomopathogen Bacillus thuringiensis as well as other human pathogens along with human disease cell lines. In particular, piceamycin potently inhibited Salmonella enterica and Proteus hauseri with MIC va
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