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Background Persistent HIV infection of long-lived resting CD4 T cells, despite antiretroviral therapy (ART), remains a formidable barrier to an HIV cure. Women have a more robust Type I interferon response during HIV infection relative to men that contributes to lower initial plasma viremia. As lower levels of viremia during acute infection are associated with reduced frequency of latent HIV infection in CD4 T cells on ART, we hypothesized that women on ART would have a lower frequency of latent HIV compared to matched men. Methods ART-