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We additionally show that Hnd catalytic properties (Km for H2, diffusion and response during the active website of CO and O2) tend to be comparable to those of standard hydrogenases (those that cannot catalyze electron bifurcation). These outcomes suggest that the clear presence of the excess subunits, necessary for electron bifurcation, modifications neither the catalytic behavior during the energetic website, nor the gas diffusion kinetics but induces unusual rates of large prospe