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The steroid hormones including brassinosteroids, regulate plant growth under stress. It is hypothesized 24-epibrassinosteroids (24-EBR) can affect safflower (Carthamus tinctorius) biochemical properties, crop yield and oil content under drought stress. The objective of our study was to determine the response of three safflower genotypes (Goldasht, Faraman and Sina) to exogenous 24-EBR (0 and 10-7 M) under drought stress including 85%, 65% and 45% of field capacity in 2015. Stress decreased chlorophyll a, b, and total, carotenoid, relat