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UCA is capable of efficiently identifying CPuORFs likely to be conserved because of the functional importance of their encoded peptides. Furthermore, our data show that the approach in which uORF sequences from multiple species are compared with those of many other species, using ESUCA, is highly effective in comprehensively identifying CPuORFs conserved in various taxonomic ranges.BACKGROUND Emergence of antibiotic resistance is a global public health concern. The relationships between antibiotic use, the gut community composition, no