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Microalgae have been recently cultivated using resources from wastewater treatment plants, including nutrients, CO2, and heat. In the present study, we focused on the natural cultivation of total microalgae rather than specific microalgae, for which culture conditions and the cultivation environment are difficult to prepare. Natural microalgal cultivation systems using 380-L raceway tanks were operated outdoors for 8 months and the effects of the culture substrate were investigated. The cultivation substrate was the primary effluent wit
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