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An important reduction (p ≤ 0.05) in inhibitory task was seen for nisin-biogel solutions stored at 37 °C, over a 24-month period, which was not observed utilizing the various other problems. The nisin-biogel revealed no cytotoxicity resistant to the cells tested at concentrations up to 200 µg/mL. CONCLUSIONS Our results verified the possibility of the nisin-biogel for canine PD control, supporting the growth of an in vivo clinical trial.Electronic medical documents