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Pollen grains are germinated in a hydrogel containing agarose and their growth is recorded in 3D making use of brightfield microscopy. Making use of ideal analysis computer software, variables such development price and pollen tube diameter are able to be determined to approximate the exerted penetration force.To achieve fertilization, pollen tubes need certainly to protect and correctly deliver sperm cells through the pistil into the ovules. Pollen tube development is a representative examp