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The first complete mitochondrial genome sequence of Metapenaeus was characterized from sand shrimp Metapenaeus ensis using shotgun assembly method. The complete mitochondrial DNA sequence is a 15 944 bp circular molecule, and contains 13 protein-coding genes, 22 transfer RNA genes, two rRNA genes, and a control region. The gene arrangements are consistent with the pancrustacean ground pattern. The phylogenetic trees were divided two clades besides outgroup, which belonged to Penaeus s.l. and Metapenaeus genus. The molecular analyses pr