https://www.selleckchem.com/pr....oducts/shp099-dihydr
The size of the neocortex and its morphology are highly divergent across mammalian species. Several approaches have been utilized for the analysis of neocortical development and comparison among different species. In the present protocol (Note This protocol requires basic knowledge of brain anatomy), we describe three ex vivo neocortical slice/tissue culture methods (i) organotypic slice culture (mouse, ferret, human); (ii) hemisphere rotation culture (mouse, ferret); and (iii) free-floating tissue culture (mouse, ferret,