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The current research had been sustained by Clovis Oncology with a grant to scholastic study organization.The present study was supported by Clovis Oncology with a grant to academic research connection. We constructed the probe 2D5-IRDye800CW by conjugated anti-CEACAM5 nanobody (2D5) with near-infrared fluorescent dye IRDye800CW. The overall performance and great things about 2D5-IRDye800CW at NIR-II had been confirmed by imaging experiments in mouse vascular and