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Municipal solid waste incineration fly ash (MSWIFA) is considered as a hazardous solid waste because of the high mobility of heavy metals. In this study, the removal of heavy metals in MSWIFA using lactic acid fermentation broth (LAF under various leaching protocols (i.e. LAFB addition amount and timing) was investigated. Results revealed that compared with that in pure lactic acid solution, the synergistic effect of various substances in LAFB was more favourable to the dissolution of heavy metals. Although the content of acid-soluble h