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4mm margin achieved a (mean±std.) sensitivity of 95.0±7.8% and specificity of 76.4±14.7%. A threshold of 81% SUV max with a 5.1mm margin achieved sensitivity of 65.1±28.4% and specificity of 95.1±5.2%. Preliminary evidence of thresholding and margin expansion of PSMA-PET images targeted at DILs validated with histopathology demonstrated excellent mean sensitivity and specificity in the setting of focal therapy/boosting and guided biopsy. These parameters can be used in a larger validation study supporting clinical translation. Prelimin