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Finally, the proposed method was applied for the determination of the molar ratio between ondansetron and chloride. A value of 1.001 ± 0.003 demonstrated that the stoichiometry of this drug with its counter-ion was 11.Two probes based on tetrahydroxanthylium unit (probe 1a) and methyltetrahydroxanthylium unit (probe 1b) were designed and synthesized for the detection of hydrazine. Probes 1a-b exhibited turn-on red emission signal and high selectivity toward NH2NH2. The response time of probe 1a to NH2NH2 was more than 60 min, while that