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All 3 types of chars showed strong retention with a poor desorption (6% in average) of OTC in synthetic hydrolyzed urine medium. CBC and NBC demonstrated both physisorption and chemisorption, whereas the OTC removal by BC was solely via physisorption. Nevertheless, CBC biochar demonstrated the best performance in adsorptive removal of OTC and nutrients in hydrolyzed human urine and its capability towards wastewater treatment. As the removal of nutrients were low, the treated urine can possibly be used as a safe fertilizer.Naphthalene sul