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The prepared sensor was successfully utilized to analyze environmental water and urine samples. The obtained results showed that the proposed method is in agreement with the HPLC method and can be used as a reliable alternative method for the analysis of flutamide.According to the solubility in the binary solvent of ethanol water, zein can be classified into α-, β-, γ-, and δ-zein, and the difference in amino acid compositions of these fractions is believed to affect their physicochemical properties and functionalities. This research co