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The present study aimed to investigate whether acute pain associated with castration and tail docking of male piglets may modulate the expression of salivary miRNAs and to explore their potential use as biomarkers. Thirty-six healthy piglets (Hermitage x Duroc) 4 days old were randomly assigned to three groups the first group (12 piglets) has been pre-treated with anesthetic and anti-inflammatory drugs (ANA) and then castrated and tail docked; the second one (12 piglets) has been castrated and tail docked without any drugs (CONV); the thi
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