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Urban surface runoff is an important non-point pollution source, and research on contamination characteristics of runoff is urgent for improving urban aquatic environment quality. Typical cities along the downstream Yangtze River, Wuxi and Nanjing, were selected in this study, and runoff samples from various underlying surfaces were collected to investigate contamination characteristics of heavy metals and dissolved organic matter (DOM). The results indicated that the concentration of heavy metals have exceed water quality standards in