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In the present study, electro-coagulation (EC) with stainless steel (SS) electrodes has been used as a pretreatment process before the reverse osmosis (RO) for the biologically treated distillery spent wash. The optimized operating parameters (pH, time, current, and electrode distance) for the EC process were obtained from the previous study. EC treated wastewater was further used as a feed for the RO system. RO membrane system operating parameters (pH, temperature, and trans-membrane pressure) were optimized by employing response surface