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© 2020 The Authors. Journal of Clinical Laboratory Analysis Published by Wiley Periodicals, Inc.desire to associated with the present study would be to measure the aftereffect of semen choice by single-layer centrifugation (SLC) carried out before freezing on sperm quality after thawing of Fleckvieh bull semen. Ejaculates from 22 bulls had been collected by artificial vagina and split into two aliquots. One aliquot (control sample) was diluted with Steridyl® and fro