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caballi) and Theileria (T. parva and T. ovis) parasites were not detected in the current study. The PCR-positive samples were confirmed by direct sequencing of the product. This study is the first to report on a relationship of Babesia parasites with tick species in Lesotho and it is evident that vector-borne diseases are present in ticks of domestic animals in this country. Research findings in this study require a joint effort from both veterinary and medical sectors to unite and conduct more epidemiological studies o