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Tumor-infiltrating immune cells play a crucial role in tumor progression and response to treatment. However, limited studies on infiltrating immune cells have shown inconsistent and even controversial results in osteosarcoma (OS). In addition, the dynamic changes of infiltrating immune cells after neoadjuvant chemotherapy are largely unknown. We downloaded the RNA expression matrix and clinical information of 80 osteosarcoma patients from the TARGET database. CIBERSORT was used to evaluate the proportion of 22 immune cell types based on g