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A novel monolithic column incorporated with Schiff base network-1 (SNW-1) was prepared for in-tube solid phase microextraction (SPME) of antiepileptic drugs. The SNW-1 nanoparticles was synthesized by a solvent thermal method and embedded in the poly(glycidyl methacrylate-ethylene glycol dimethacrylate) (GMA-EGDMA) monolithic column by in situ polymerization. The amount of SNW-1 in polymerization solution for preparation of monolithic column was optimized. Fourier transform infrared spectrometry and scanning electron microscope were use