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The fluorescence quenching of CHOF-1 brought on by 4-NP is attributed into the absorption competition quenching (ACQ) procedure. When you look at the framework of CHOF-1, the hydroxyl of TNP has powerful acidity and may develop powerful hydrogen bonds because of the unreacted NH2. Then, the crystalline structure of CHOF-1 decomposed as well as the electron framework regarding the photophore changed; thus, the fluorescence spectra red-shifted. That is a novel example of a covalent rela
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