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In vitro experiments revealed that TiMNPs 100 were significantly exceptional in their power to down-regulate the appearance amount of proton-sensing receptors and crucial proteins into the PLC/Ca2+ signal path, which in turn marketed MSC osteogenesis differentiation. An increased level of ALP task, mineralization capacity and collagen secretion on TiMNPs 100 was confirmed when compared with those of other teams. This study provides an innovative new insight into creating