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Further, conserved motifs exclusive to functionally characterized MLOs were identified in MnMLO1C, MnMLO2 and MnMLO6A proteins. Combined analysis of the phylogenetic relationship, conserved motif analysis and gene expression in response to infection identified MnMLO2 and MnMLO6A as potential candidate genes involved in powdery mildew susceptibility in mulberry. Identification and deployment of natural and induced mutations in the candidate genes can be useful for mulberry breeding programs to develop powdery mildew resistant varieties.An