https://www.selleckchem.com/peptide/apamin.html
Because fluorescence staining is not sensitive in zebrafish embryos, it is replaced with diaminobezidine (DA staining here. In this protocol, HHT patient-derived iPSC lines containing an eng mutation are differentiated into endothelial cells. After coating a plate with basement membrane matrix for 30 min at 37 °C, iPSC-ECs are seeded as a monolayer into wells and kept at 37 °C for 3 h. Then, the tube length and number of branches are calculated using microscopic images. Thus, with this improved WISH protocol, it is shown that reduced en
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