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Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley Sons Ltd.Antiretroviral fixed-dose-combination drugs are best assayed with HPLC, or LC-MS-MS. However, most scientists in developing nations have no access to these expensive instruments. A more affordable quantitative technique is the use of UV-Visible spectroscopy - where often the absorption spectra of these antiretrovirals are overlapping; thus complex derivative methodologies are required for quantification.