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99339 and 0.95916, respectively). Besides, the introduction of Au-Ag ANCs makes the Raman spectra intensity of thiram (a pesticide) with a concentration of 30 ppm on the surface of the blank ITO glass significantly enhanced, and it can detect thiram with a concentration as low as 0.03 ppm. In addition, Au-Ag ANCs substrate exhibits great uniformity and stability, so they have considerable application potential in the field of quantitative detection of trace substances.We investigate in detail the azimuthal and radial modulation (i.e., t