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Overall, stratified CycleGAN generated higher quality synthetic images than traditional CycleGAN. Evaluation of cell detection accuracy confirmed that synthetic images were faithful to ground truth images of the same cells. Across this challenging dataset, F1-score improved from 76.9 ± 5.7% when using traditional CycleGAN to 85.0±3.4% when using stratified CycleGAN. These findings demonstrate the potential of stratified Cycle-GAN to improve the synthesis of medical images that exhibit a graded variation in image quality.Traditional inten