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spot at HBoV1 capsid position 590 that has accumulated in two patients during natural infection and that lowers viral loads but increases vector yields. Thereby, our study expands our current understanding of HBoV1 biology in infected human subjects and concomitantly provides avenues to improve AAV/HBoV1 gene transfer vectors. Copyright © 2020 American Society for Microbiology.Ebola virus (EBOV) continues to pose a significant threat to human health as evidenced by the 2013-2016 epidemic in West Africa and the ongoing