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The effect of green feed on health-promoting biomolecules in milk was examined in dairy buffaloes. Buffaloes received a total mixed ration (TMR) (Control, C; n = 4 or TMR + alfalfa green feed (30% of diet) (Treated, T; n = 4. Biomolecules and functional activity were measured in milk obtained twice-monthly. Treated buffaloes had higher milk l-carnitine, acetyl-l-carnitine, propionyl-l-carnitine and δ-valerobetaine (P less then 0.01). They also had higher antioxidant activity (P less then 0.01). Compared with C buffaloes, milk of T b