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In addition, a supervised classification model which based on MS spectra, was used to classify seven typical hypoglycemic polysaccharides with excellent sensitivity, specificity and accuracy, indicating the good classification performance of the RF model constructed. Thus, the proposed mass spectroscopic method provides a cost effective, accurate and high throughput tool for identification and classification of polysaccharides, which is beneficial for studying the biological activity of polysaccharides.Metal-organic frameworks (MOFs) a