https://mmpsignaling.com/index.....php/genetic-and-epi
Apoptosis was measured with flow cytometry. We also transfected A549 cells with an microribonucleic acid-21 (miR-21) mimic or negative control ribonucleic acid (RNA) duplex and phosphatase and tensin homolog, erased on chromosome 10 (PTEN) small interfering ribonucleic acid (siRNA) or unfavorable control. PTEN, phosphorylated protein kinase B (pAKT), and necessary protein kinase B (AKT) appearance had been detected utilizing Western blotting, whereas miR-21 expression had been analyzed
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