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304 and = 0.136, respectively). Of the 317 eyes, 108 (34.1%) showed no bacterial growth in the pre-preparation period, which was similar in the three groups. was the most common isolated bacteria. Conjunctival injection was significantly different among studied groups ( = 0.0001), five patients in iodine group had severe conjunctival injection and no one in the other group. SPE was significantly fewer in chlorhexidine group than PHMB and iodine groups ( = 0.0001). Pretreatment with 5% Povidone-Iodine (PVI) for at least 15 min or repeate